?實驗小鼠是進行科學(xué)研究常用的實驗動物，小鼠不僅和人的基因具有相似度，而且具有體型小善謀新篇、易飼養(yǎng)探索、生長繁殖快、操作管理方便的優(yōu)點先進的解決方案。隨著小鼠在科學(xué)研究中的廣泛應(yīng)用拓展，科研人員培育出了許多小鼠品種及品系創造更多。在進行研究計劃時，選擇合適品系的小鼠建立動物疾病模型不斷進步，是實驗設(shè)計的關(guān)鍵基礎(chǔ)一步工藝技術。

BALB/c小鼠?：

?遺傳背景?：1913年美國國立腫瘤研究所培育，經(jīng)過多代近交繁殖規模。

?特征?/用途?：?

1.性格溫順近年來，易于繁殖，雌雄體重差異邪l展目標奮鬥〖夹g先進；

2.乳腺腫瘤發(fā)病率低(3％)，當用乳腺腫瘤病毒(MTV)誘導(dǎo)時發(fā)病率將增高作用。對礦物油誘導(dǎo)漿細胞瘤敏感情況正常，廣泛應(yīng)用于雜交瘤和單克隆抗體的生產(chǎn)；

3.易患慢性肺炎技術特點；

4.有自發(fā)高血壓癥提高鍛煉，老年鼠心臟病變，雌雄鼠均有動脈硬化凝聚力量，幾乎全部20月齡的雄鼠脾臟均有淀粉樣變有所提升；

5.對放射線敏感，應(yīng)用于核醫(yī)學(xué)新的力量。

?C57BL/6小鼠?：

?遺傳背景?：1921年C.C.Little用Abby Lathrop小鼠培育而成先進水平。

?特征?/用途?：

1.已經(jīng)完成基因組測序的小鼠品系；

2.品系穩(wěn)定全面展示、容易繁殖越來越重要的位置；

3.試驗結(jié)果精度高，可比性好共同學習，應(yīng)激反應(yīng)均一順滑地配合；

4.構(gòu)建基因修飾動物模型，可保證遺傳背景的高度穩(wěn)定性和實驗數(shù)據(jù)一致性效高。

有了這些基礎(chǔ)知識背景前沿技術，我們在構(gòu)建模型時，設(shè)計實驗時性能，參考文獻時多種方式，評價數(shù)據(jù)時，就能更準確技術創新。尤其我們使用荷蘭Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸鹽脂質(zhì)體清除巨噬細胞時深入交流研討，我們除了關(guān)注這些因素，還需要考慮小鼠的周齡。巨噬細胞的高度異質(zhì)性和可塑性需求，也覺得巨噬細胞的多功能性和多面性堅定不移，動態(tài)性和適應(yīng)性。比如巨噬細胞可以質(zhì)控造血干細胞（見Science文獻）更讓我明白了，巨噬細胞可以和神經(jīng)元一樣調(diào)控運動（見Natuer文獻）迎難而上。在這些頂刊的重大研究發(fā)現(xiàn)中，研究人員都使用荷蘭Liposoma的巨噬細胞清除劑（CP-005-005）貨號探索。此外堅持先行，巨噬細胞等免疫細胞，在不同品系滿意度，不同周齡情況較常見，不同性別，也是差異很大主要抓手。見如下這篇文獻的基礎(chǔ)研究體製。

小鼠選擇

品系：C57BL/6和BALB/c

性別：雄性鼠

周/月齡：1、3創新科技、5服務延伸、10和18月齡

處死方式：通過CO2窒息處死

樣品：外周血和脾臟

流式細胞術(shù)分析

1、樣本制備:

外周血單個核細胞(PBMC):血液PBS稀釋后,使用ACK裂解液去除紅細胞•脾細胞:機械分離后同樣使用ACK裂解液處理

2具有重要意義、抗體標記: 使用熒光標記的單克隆抗體組合:

FITC標記: CD4進一步、CD19、MHC II應用創新、CD11b

PE標記: CD3提高、CD8、CD11c的特性、CD44

PerCP標記: CD3交流、CD19

研究結(jié)果

參考文獻：

Pinchuk LM, Filipov NM. Differential effects of age on circulating and splenic leukocyte populations in C57BL/6 and BALB/c male mice. Immun Ageing. 2008 Feb 11;5:1. doi: 10.1186/1742-4933-5-1. PMID: 18267021; PMCID: PMC2268915.

Results

Using a multipoint age comparison approach, cells from the two major immune system compartments, peripheral blood and spleen, and flow cytometry analysis, we found several principal differences in T cell and professional antigen presenting cell (APC) populations originating from a prototypical T helper (Th) 1 mouse strain, C57BL/6, and a prototypical Th2 strain, BALB/c. For example, regardless of age, there were strain differences in both peripheral blood mononuclear cells (PBMC) and spleens in the proportion of CD4+ (higher in the BALB/c strain), CD8+ T cells and CD11b+/CD11c+ APC (greater in C57BL/6 mice). Other differences were present only in PBMC (MHC class II + and CD19+ were greater in C57BL/6 mice) or differences were evident in the spleens but not in circulation (CD3+ T cells were greater in C57BL/6 mice). There were populations of cells that increased with age in PBMC and spleens of both strains (MHC class II+), decreased in the periphery and spleens of both strains (CD11b+) or did not change in the PBMC and spleens of both strains (CD8+). We also found strain and age differences in the distribution of na?ve and memory/activated splenic T cells, e.g., BALB/c mice had more memory/activated and less naive CD8+ and CD4+ T cells and the C57BL/6 mice.

Conclusion

Our data provide important information on the principal differences, within the context of age, in T cell and professional APC populations between the prototypical Th1 mouse strain C57BL/6 and the prototypical Th2 strain BALB/c. Although the age-related changes that occur may be rather subtle, they may be very relevant in conditions of disease and stress. Importantly, our data indicate that age and strain should be considered in concert in the selection of appropriate mouse models for immunological research.